アブストラクト（10巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Delineation of Cell Surfaces Using Modified Ruthenium Red Staining and Electron Microscopy
Subtitle　： ORIGINAL ARTICLES
Authors　： Tsuneo Takahashi, Kazuto Takahashi, Janet Hock*
Authors（kana）　：
Organization　： Department of Oral Anatomy, Kanagawa Dental College, *Department of Periodontology, University of Connecticut Health Center
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 10
Number　： 2
Page　： 117-121
Year/Month　： 1982 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The use of ruthenium red as a stain for electron microscopy of external cell surfaces was tested using cultured rat osteosarcoma cells, ROS/17. In situ staining of cell cultures with osmium tetroxide and 0.01, 0.05 or 0.1% ruthenium red, which had been left to oxidize for 3 hours prior to use, resulted in well defined cell boundaries. The stain penetrated consistently into the closely opposed intercellular junctional areas. If section staining by uranyl acetate-lead citrate is also done, intracellular components can be identified. This clear definition of extra-and intracellular surfaces will facilitate quantitation of the cell ultrastructure.
Practice　： Dentistry
Keywords　： Ruthenium Red, Culture, Electron Microscopy