アブストラクト（16巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Effect of Epidermal Growth Factor on Protein Synthesis in Human Alveolar Bone Cells
Subtitle　： ORIGINAL ARTICLE
Authors　： Makoto Fujieda
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 16
Number　： 2
Page　： 67-73
Year/Month　： 1988 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The purpose of this study was to investigate the ability of cultured human alveolar bone (HAB) cells to produce proteins and collagen synthesis when stimulated by the epidermal growth factor (EGF). The HAB cells were plated on 60-mm tissue culture dishes at a density of 1.5 × 105 cells/5 ml medium, and cultured for 4 days until confluent. The cells were then transferred to a medium supplemented with 2 percent calf serum containing varying concentrations of EGF and were further cultured for 4 days. A 24-hour treatment of the HAB cells with EGF in doses ranging from 10-100 ng/ml stimulated the incorporation of (3H) thymidine and DNA synthesis in a dose-dependent manner. This short-term stimulatory effect was sustained in a long-term culture with a dose-dependent increase in the cell proliferation by the HAB cells. A lag period of 8 hr occurred before significant stimulation of (3H) thymidine incorporation was observed. Commitment to increased incorporation of (3H) thymidine required a minimum of 6-hr continuous incubation with EGF. The assessment of protein and collagen synthesis was performed on HAB cell cultures that were pulsed for 3hr with 10 uci of L-(3H)-proline in the presence (test) and the absence (control) of 10ng/EGF per ml, 95 percent air/5 percent CO2 atmosphere. The radioactivity of the proteins in the supernatant solubilized by collagenase was measured to determine the collagen synthesis. EGF at concentrations of 3 to 50 ng/ml significantly decreased the collagen synthesis in the cells, whereas the protein synthesis was stimulated. This hormone also caused a dose-related suppression of the hydroxyproline content which was detectable 2 days after an EGF addition. These results indicate that EGF at concentrations of 10-50 ng/ml significantly decreased the collagen synthesis in the cells, whereas the protein synthesis was rather stimulated. Thus, the proportion of collagen to protein synthesized decreased markedly with increasing concentrations of EGF. It is apparent that EGF has a profound adverse effect on collagen metabolism of HAB cell cultures which may explain the loss of collagen observed in the bone cells in vitro.
Practice　： Dentistry
Keywords　： Alveolar bone, EGF, DNA synthesis, Collagen, Osteoblasts, Calcification