アブストラクト（16巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Physical Stress Increased Alkaline Phosphatase Activity in Cultured Bone Cells
Subtitle　： ORIGINAL ARTICLE
Authors　： Takao Kubota, Masato Yamauchi, Makoto Takeuchi, Iwao Kinoshita, Sadao Sato, Yoshii Suzuki
Authors（kana）　：
Organization　： Department of Orthodontics, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 16
Number　： 2
Page　： 75-80
Year/Month　： 1988 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The effect of mechanical stretching on an alkaline phosphatase (ALPase) activity was studied by bone cell culture. Primary cultures of bone cells, isolated from a 2-day-old rat calvaria, were subcultured in dishes with a flexible plastic bottom. The attached cells were stretched by means of the method described by Hasegawa et al., 1985. The ALPase activity was increased by an intermittent stretching of confluent cells. The results of SDS-PAGE showed that the increased ALPase activity raised in the 110K band. This stretch-responsive ALPase was bound to hyaluronic acid. Hyaluronic acid synthesis increased in the stretched cells. These results indicate that the mechanical stretch-sensitive ALPase is a hyaluronic acid binding, 110K ALPase.
Practice　： Dentistry
Keywords　： Alkaline Phosphatase Activity, Bone Cell, Mechanical Stretching