アブストラクト（18巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Isolation and Partial Characterization of Bovine Periodontal Ligament Alkaline Phosphatase
Subtitle　： ORIGINAL ARTICLES
Authors　： Sadao Sato, Toshio Kawase*, Takao Kubota, Shigeru Saito*
Authors（kana）　：
Organization　： Department of Orthodontics, Kanagawa Dental College, *Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 18
Number　： 1
Page　： 43-53
Year/Month　： 1990 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] An alkaline phosphatase which binds to hyaluronate with a high affinity has been extracted from the bovine periodontal ligament and purified. The procedure consisted of extraction with a 10 mM Tris/Mg buffer containing 0.1% Nonidet P40, chromatography on a hyaluronate-conjugated Affi-Gel 15, and fast protein liquid chromatography. The hyaluronate-binding alkaline phosphatase has an apparent molecular weight of 110,000, contained sialic acid and was more stable to heat treatment than were two other species (Mr=120,000 and 130,000) extracted from PDL. The heat stability and influence of inhibitors on its activity show that the hyaluronate-binding alkaline phosphatase of periodontal ligament was similar to the bone alkaline phosphatase.
Practice　： Dentistry
Keywords　： Alkaline Phosphatase, Periodontal Ligament, Hyaluronate