アブストラクト（19巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： High 5α-Reductase Activities in 7,12-Dimethylbenz[a]anthracene Induced Submandibular Gland Cancer
Subtitle　： ORIGINAL ARTICLES
Authors　： Akihiko Amaki, Yasumasa Kato, Shinri Koshika
Authors（kana）　：
Organization　： Department of Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 19
Number　： 2
Page　： 67-75
Year/Month　： 1991 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Testosterone (T) metabolism and the effects of sex hormones on the growth were investigated in the squamous carcinoma (RSS) cell strain of the rat submandibular gland (SMG) induced by 7,12-dimethylbenz[a]anthracene (DMBA). A large amount of dihydrotestosterone (DHT) was produced from the substrate T in the RSS cell. The higher activity of 5α-reductase was revealed in the RSS than normal cells. The DHT formations from the substrate were 8.3pmol/l×105 cells for normal cells and 17.5pmol for the RSS cells, when 3H-T was incubated at the concentration of 7.2μM for 20 min. The DHT formation in sarcoma (RFI) cell was similar to normal. Δ4-Androstenedione (Δ4-Dione) production, however, was lower in the RSS than normal. The growth of RSS cells was accelerated at the T concentration of 10-7μM and the growth showed a plateau at 10-4μM, showing 1.8-fold on nontreated cells. The growth of the cells was increased 1.5-fold by 10-4μM of estradiol-17β (E2) as compared with nontreated cells. The cell growth was not influenced by progesterone (P4). These results mean that T metabolism exists in the carcinoma possessing high 5α-reductase activities in the cells and that the growth of the tumor cells is controlled by androgen.
Practice　： Dentistry
Keywords　： 5α-Reductase, Submandibular Gland, Cancer