アブストラクト（24巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Expression of Trypsinogen mRNA in Squamous Cell Carcinoma Cell Line from 7, 12-Dimethylbenz[a]anthracene-Induced Rat Submandibular Gland
Subtitle　： SHORT COMMUNICATION
Authors　： Yasumasa Kato, Satoru Ozono1, Shinri Koshika
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College, 1Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 24
Number　： 1
Page　： 15-18
Year/Month　： 1996 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] A squamous cell carcinoma cell line (RSS18) derived from a 7, 12-dimethylbenz[a]anthracene-induced submandibular gland of a femal rat was tested for production of ECM-degradative proteinases. Zymographic analysis showed that gelatinolytic activities associated with 85-and 25-kDa proteins were secreted in a serum-free medium. Enzyme-inhibition assays were performed to determine the proteinase type. The gelatinolytic activities of the 85-and 25-kDa proteins were completely inhibited by diisopropyl fluorophosphate, suggesting that they belong to the serine proteinase type. When the conditioned medium was treated with enteropeptidase at 37℃ for 10min before zymographic analysis, the gelatinolytic activity of the 25-kDa protein significantly increased. Moreover, reverse transcription-polymerase chain reaction analysis revealed that trypsinogen mRNA was expressed in the cells. These results suggested that 85- and 25-kDa proteins were similar to plasmin and trypsin proteinases, respectively.
Practice　： Dentistry
Keywords　： Trypsinogen, Squamous cell carcinoma, Submandibular gland, DMBA