アブストラクト（25巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Morphological Study on Cell Death Induced by Radiation and Anticancer Agents
Subtitle　： ORIGINAL ARTICLES
Authors　： Yasuhumi Nagata, Keiichi Tsukinoki, Yoshihisa Watanabe
Authors（kana）　：
Organization　： Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 25
Number　： 2
Page　： 73-82
Year/Month　： 1997 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] This study was designed to confirm the involvement of apoptosis in the morphological changes of cells induced by gamma ray irradiation and anticancer agent (vinblastine sulfate) in the human-derived oral squamous cell carcinoma cell line KB. To detect the apoptosis, we employed three-dimensional analysis of karyomorphism using confocal laser scanning and transmission electron microscopies and the Tunel method. By gamma rays irradiation, the induction of apoptosis was recognized morphologically. The Tunel-positive cell ratio indicated that apoptosis was induced at the highest rate 96 hrs after irradiation (1Gy, 5Gy, 10Gy, 20Gy and 50Gy). Vinblastine sulfate induced apoptosis in the morphological context. These results suggested that apoptosis took part in the mechanism of tumor cell shrinkage which was affected by radiotherapy and chemotherapy.
Practice　： Dentistry
Keywords　： Apoptosis, Chemotherapy, Radiotherapy, Karyomorphism, Three-dimensional analysis