アブストラクト（26巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Lectin-Dependent Cellular Cytotoxicity of Activated Killer Cells Derived from PBMC or TIL to Autologous Tumour Cells
Subtitle　： ORIGINAL ARTICLES
Authors　： Yuuichi Sasakura, Chinatsu Ibuki, Shun-suke Yanoma1), Gouichi Matsumoto, Yasushi Ohmi, Jun-ichi Shindo
Authors（kana）　：
Organization　： First Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 1)Clinical Research Centre, Kanagawa Cancer Centre
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 26
Number　： 2
Page　： 69-73
Year/Month　： 1998 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Interleukin-2 (IL-2) with anti-CD3 antibody can induce numerous lymphokine activated killer (CD3LAK) cells. However, CD3LAK cells exhibit less cytotoxicity than LAK cells treated with IL-2 alone. We estimated the enhancement of cytotoxicity by PHA or PWM against peripheral blood mononuclear cells (PBMC)-CD3LAK cells derived from peripheral blood mononuclear cells from a clinical healthy volunteer as well as PBMC-CD3LAK and tumour infiltrated lymphocytes (TIL)-CD3LAK cells obtained from two patients with tongue cancer (stage II). PHA or PWM added for assay period (4 hours) of cytotoxicity in microplate. Cytotoxicity was estimated with a 51Cr release assay. We found that NK activity of the PBMC-CD3LAK cells derived from a clinical healthy volunteer was significantly enhanced by PHA or PWM. LAK activity of the above cells or PBMC-CD3LAK cells derived from case 2 was enhanced significantly only by PWM. The NK activity of TIL-CD3LAK cells derived from case 1 was enhanced by PHA or PWM, and the LAK activity of these cells was enhanced significantly by PWM. The LAK activity of TIL-CD3LAK cells derived from case 2 was enhanced by PWM. As for cytotoxicity against the autologous tumour, PBMC-CD3LAK cells derived from case 2 were also slightly enhanced by PHA or PWM, but TIL-CD3LAK cells enhanced significantly more than the PBMC-CD3LAK cells in this study. These results suggest differences between PBMC-CD3LAK and TIL-CD3LAK cells in the repertoire and in the sugar chain expressed on the tumour cell surface.
Practice　： Dentistry
Keywords　： Lectin-dependent cellular cytotoxicity, Oral cancer, Autologous tumour, Lymphokine activated killer cell