アブストラクト（27巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Role of LFA-1 in the Osteoclast Formation
Subtitle　： Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998
Authors　： Nobuyuki Tani-Ishii, Akira Tsunoda, Gouichi Matsumoto1), Toshio Teranaka, Toshio Umemoto2)
Authors（kana）　：
Organization　： Department of Operative Dentistry and Endodontics Kanagawa Dental College, 1)Department of Oral surgery, Kanagawa Dental College, 2)Department of Oral Microbiology Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 27
Number　： 1
Page　： 79-81
Year/Month　： 1999 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] The leukocyte integrin LFA-1 (CD11a/CD18) plays an important role in lymphocyte recirculation and homotypic interactions. Recently, it has been reported that LFA-1 and ICAM-1 are involved in the formation of osteoclast. In this study, the role of LFA-1 in osteoclast formation via interaction between stromal cells and osteoclast progenitors and/or among osteoclast progenitors was investigated. Osteoclast formation was observed on coculture of LFA-1 deficiency mouse (CD11a-/-) bone marrow cells and MC3T3-G2/PA6 cells on a dentin slice in the presence of 1α,25(OH)2D3 and dexamethasone for 7 days. At the end of culture period, the number of TRAP-positive cells was counted and the resorption pits area were evaluated by reflected light microscopy. The number of TRAP-positive cells was significantly decreased (176.4+-11.7 vs 260.0+-20.1 (control: C57BL/6N) cells/1x106μm2) and the total pits area per dentin slice was also decreased (79,911+-1,075 vs 113,236+-9,818 (control: C57BL/6N)μm2/3x105μm2) in CD11a-/- mouse. On the other hand, the ratio of TRAP-positive mononuclear to multinuclear cells was not changed. These results show that adhesion molecules LFA-1 may play a role in osteoclast formation via interaction between stromal cells and osteoclast progenitors.
Practice　： Dentistry
Keywords　： LFA-1 deficiency, Osteoclast, MC3T3-G2/PA6