アブストラクト（31巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Evidence of Cytokine and Matrix Degrading Enzyme Induction by Mycoplasma fermentans-Derived Lipoprotein in Blood Cells and Synoviocytes
Subtitle　： ORIGINAL ARTICLES
Authors　： Masako Suga1), Nobushiro Hamada2), Eiro Kubota1)
Authors（kana）　：
Organization　： 1)Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 2)Department of Oral Microbiology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 31
Number　： 2
Page　： 151-159
Year/Month　： 2003 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Although Mycoplasma fermentans(M.fermentans)is a human pathogen suspected to play a role in inducing arthritis, its pathogenic mechanisms are poorly understood. We undertook the present study to find out whether protein derived from M.fermentans(TXLP)can stimulate cells composed of articular tissue. We used mouse peritoneal macrophages(PEC), a rabbit synoviocyte cell line(HIG-82), normal human articular chondrocytes(NHCKn), a cell line derived from a human chondrosarcoma(USAC), and a human myelomonocytic cell line(THP-1)in the experiments. TXLP induced PEC and THP-1 cells to produce significantly more TNF-α and IL-1β than was produced by unstimulated control cells. TXLP stimulation also induced HIG-82 to express matrix metalloproteinase-3(MMP-3)mRNA. Two Tolllike receptors, TLR2 and TLR4, were expressed on THP-1, but USAC and NHC-Kn showed no evidence that either TLR2 or TLR4 was synthesized and there was no evidence that the gene for either was transcribed. These results show that antigen derived from M.fermentans stimulates blood cells to secrete cytokines, which play an important role in the development of arthritis, and it also stimulates rabbit synoviocytes to express more MMP-3 mRNA. We hypothesize that the presence of M.fermentans or its antigen in the TMJ increases the susceptibility of the TMJ to mechanical damage by exacerbating inflammation in TMJ tissue.
Practice　： Dentistry
Keywords　： Temporomandibular disorders, Mycoplasma Fermentans, Articular cells, Cytokines, Matrix degrading enzymes