アブストラクト（31巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Induction of IL-1β production and osteoclast differentiation by P.gingivalis minor fimbriae
Subtitle　： Selective Proceedings of 37th General Meeting of Kanagawa Odontological Society, 2002
Authors　： Hiroko Hiramine, Kiyoko Watanabe, Nobushiro Hamada, Toshio Umemoto
Authors（kana）　：
Organization　： Department of Oral Microbiology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 31
Number　： 1
Page　： 65-67
Year/Month　： 2003 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： The effects of minor fimbriae of Porphyromonas gingivalis which consisted of 67-kDa fimbrillin on IL-1β production in murine peritoneal macrophages and induction of osteoclast differentiation were investigated. Murine peritoneal macrophages were stimulated with 67-kDa minor or 41-kDa major fimbriae, and the levels of IL-1β production were determined by ELISA. To estimate osteoclast differentiation, mouse osteoclast precursors were placed on dentine slices, and cultured with or without fimbriae for 7 days. Both minor and major fimbriae explicitly induced IL-1β production in murine macrophages. Furthermore, pit formations on the dentin slices were significantly enlarged when the osteoclast precursors were incubated with P.gingivalis fimbriae. Pretreatment of murine macrophages with anti-TLR2 and/or anti-TLR4 antibodies inhibited IL-1β activity stimulated with both fimbria. These results suggest that minor fimbriae of P.gingivalis may provoke host inflammatory response as well as major fimbriae and be involved in the development of periodontal disease.
Practice　： Dentistry
Keywords　： Porphyromonas gingivalis, Fimbriae, cytokine, osteoclast