アブストラクト（8巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： The Metabolic Studies of Succinate-1,4-14C in Bovine Periodontal Ligament - Preparation and Determination of Organic Acids, Amino Acids and Other Low Molecular Intermediates
Subtitle　： ORIGINAL ARTICLES
Authors　： Toshio Kawase
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 8
Number　： 1
Page　： 83-95
Year/Month　： 1980 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The biosynthetic metabolites of freshly extracted bovine periodontal ligaments have been studied in terms of 14C incorporation into relatively low molecular intermediates from succinate-1,4-14C added as radioactive substrate to the short term incubation systems. The results obtained in this experiment demonstrate that carbon atoms existing in 1 and 4 position of the succinate convert not only into organic acids such as fumarate and malate but also, with highly active transamination, synthesize into mainly acidic amino acids. The different linear functions for carbon dioxide production in the bovine periodontal ligament, pulp and liver indicate evidently the independent properties of their cellular activities in regard to oxidative phosphorylation. Moreover, it is particularly note worthy for the discovery of unidentified material in the periodontal ligament. This material showed rapid linear increase of radioactivity indicating accumulation of 14C from succinate-1,4-14C and exhibited similar absorption spectra with a neutral salt extract of periodontal ligament. Therefore, the material may be of physiological importance and its identification may, in terms of metabolism, help to explore cellular specificity in the periodontal ligament.
Practice　： Dentistry
Keywords　： Bovine periodontal ligament, Short-term incubation method, Succinate-1,4-14C, Metabolic specificity