アブストラクト（9巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Adsorbed Organic Compound by Apatite : Heterogeneity of Adsorption Peptides on CNBr Peptides of α1 (I) Chain from Periodontal Ligament Collagen
Subtitle　： ORIGINAL ARTICLES
Authors　： Katsuhiro Nishiyama
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 9
Number　： 2
Page　： 53-66
Year/Month　： 1981 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] This investigation showed that CNBr peptides from α1 (I) collagen of bovine periodontium were adsorbed to the surface structure of hydroxyapatite. These peptides were then used as a model system to study nucleation catalysis for apatite precipitation in metastable solution. The CNBr peptides of α1 (I) chain which were purified by CM-chromatography were adsorbed on a hydroxyapatite column. The peptides were eluted from hydroxyapatite by a stepwise process which increased the concentration of 0.001M, 0.01M, and 0.1M phosphate ions. The peptides exhibited the ability to maintain various solubilities of the precipitating calcium phosphate and to inhibit hydroxyapatite crystal growth. The α1 (I) chains, purified by CM-cellulose chromatography, were also subjected to amino acid analysis for comparison with α1 (I) collagen of the gingiva and skin. The CM-cellulose chromatogram for resolution of the CNBr peptides was characteristic of collagens which are rich in α1 (I) form. The binding of CNBr peptides to hydroxyapatite may be based on negatively charged groups on the macromolecules which have an affinity to ionic calcium on the hydroxyapatite. When eluted, the CNBr peptides (especially α1-CB3, α1-CB7, and α1-CB4) indicated the weakest affinity to hydroxyapatite. However, the α1-CB8 and α1-CB6 peptides were released at a concentration of 0.1M phosphate buffer. Although the fraction richest in α1-CB8 has the highest activity, it is possible that this may contribute to nucleation catalysis in apatite formation by CNBr peptides from α1 (I) collagen of bovine periodontium.
Practice　： Dentistry
Keywords　： Hydroxyapatite, Collagen, Calcification, Periodontium, α1 (I) chain