アブストラクト(10巻1号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Organic Matrix Biosynthesis by Surviving Alveolar Bone in Vitro
Subtitle : ORIGINAL ARTICLES
Authors : Sadao Sato, Shin Hasegawa, Teruaki Kamei, Masazumi Ooshita, Yoshii Suzuki, Yasuyuki Enomoto*, Shigeru Saito*
Authors(kana) :
Organization : Department of Orthodontics, Kanagawa Dental College, *Department of Oral Biochemistry, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 10
Number : 1
Page : 1-8
Year/Month : 1982 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] Twenty male Japanese white rabbits were used to examine the biosynthesis of organic matrix components in alveolar bone cells. The alveolar bones were removed, incubated with radioactive succinate as pulse incubation for 30 minutes and then incubated with unlabeled succinate as chase incubation for 30, 60, 120 and 240 minutes at 37℃. After chase incubation, the intracellular components, extracellular components, EDTA extractable matrix components, TCA soluble matrix components, and the insoluble matrix components were fractionated from the incubation medium and alveolar bone tissue. Each fraction was subjected to gel chromatography and amino acid analysis. The results suggested that the alveolar bone cells actively produced collagen and non-collagenous proteins in vitro and that the newly synthesized organic components were rapidly released in the extracellular medium. Furthermore, these molecules were converted into matrix components such as an insoluble aggregate, especially collagenous components. The chase incubation technique using radioactive succinate made it possible to explore the biosynthesis of organic matrix components in alveolar bone tissue.
Practice : Dentistry
Keywords : Alveolar Bone, Organic Matrix Synthesis, Chase Incubation, Radioactive Succinate