アブストラクト(10巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Delineation of Cell Surfaces Using Modified Ruthenium Red Staining and Electron Microscopy
Subtitle : ORIGINAL ARTICLES
Authors : Tsuneo Takahashi, Kazuto Takahashi, Janet Hock*
Authors(kana) :
Organization : Department of Oral Anatomy, Kanagawa Dental College, *Department of Periodontology, University of Connecticut Health Center
Journal : The Bulletin of Kanagawa Dental College
Volume : 10
Number : 2
Page : 117-121
Year/Month : 1982 / 9
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] The use of ruthenium red as a stain for electron microscopy of external cell surfaces was tested using cultured rat osteosarcoma cells, ROS/17. In situ staining of cell cultures with osmium tetroxide and 0.01, 0.05 or 0.1% ruthenium red, which had been left to oxidize for 3 hours prior to use, resulted in well defined cell boundaries. The stain penetrated consistently into the closely opposed intercellular junctional areas. If section staining by uranyl acetate-lead citrate is also done, intracellular components can be identified. This clear definition of extra-and intracellular surfaces will facilitate quantitation of the cell ultrastructure.
Practice : Dentistry
Keywords : Ruthenium Red, Culture, Electron Microscopy