アブストラクト（11巻1/2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Metabolism in Mitochondrial Fractions Isolated from Bovine Periodontal Ligament, Bovine Dental Pulp and Mouse Liver - A Comparison with Whole-Cell Preparations -
Subtitle　： ORIGINAL ARTICLES
Authors　： Shinichi Yokomizo, Chiyoe Meguro, Kenichi Sugizaki, Tsunekazu Makino, Masato Nakajima, Toshio Kawase, Shigeru Saito
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 11
Number　： 1/2
Page　： 5-14
Year/Month　： 1983 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Bovine periodontal ligament or bovine dental pulp was incubated 60 min in Krebs-Ringer phosphate buffer with succinate-1,4-14C as radioactive substrate for the whole-cell preparation. The mitochondrial fraction, after isolation by differential centrifugation, was suspended in phosphate sucrose buffer and incubated for 30 min. Subsequent newly-synthesized organic and amino acids were isolated by centrifugation, eluted by Dowex 50 column and measured for radioactivity by liquid scintillation. Production of organic acids such as malate, fumarate and α-ketoglutarate was compared, whole cell vs subcellular. Major production of amino acids after whole-cell incubation was of glycine, alanine, glutamate and aspartate, and after mitochondrial fraction incubation, glutamate, glycine, alanine and aspartate comprised the major production. Proline production was minor. Thus, synthetic pathways from the tricarboxylic acid cycle intermediates to amino acids were shown to operate in vitro after incubation of either whole-cell preparation or mitochondrial fraction with succinate as the accompanying substrate.
Practice　： Dentistry
Keywords　： Periodontal Ligament, Mitochondria, Succinate, TCA Cycle