アブストラクト（14巻1/2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Proteoglycan Isolated from Rabbit Alveolar Bone
Subtitle　： ORIGINAL ARTICLES
Authors　： Tomoko Oosawa
Authors（kana）　：
Organization　： Department of Orthodontics, Kanagawa Dental College, Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 14
Number　： 1/2
Page　： 17-25
Year/Month　： 1986 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The isolation and characterization of a proteoglycan associated with the mineral phase of a rabbit alveolar bone was undertaken in order to contribute to the understanding of bone remodeling. The isolation of the proteoglycan was achieved by the formic acid precipitation of macromolecules extracted during demineralization of the bone matrix followed by subsequent procedures involving gel sieve chromatography on Sepharose CL-6B, ion-exchange chromatography on DEAE-Sephacel and rechromatography on Sepharose CL-6B under dissociative conditions. The isolated proteoglycan with Kav=0.35 (Sepharose CL-6B) was observed as a broad band stained with Alcian blue after SDS polyacrylamide gel electrophoresis. It was composed of 49.1% protein, 25.3% hexosamine, 29.2% hexuronic acid and 6.0% sialic acid. The core protein which was obtained after digestion of the proteoglycan with chondroitinase ACII had an apparent Mr=48,000 (SDS-PAGE). In addition, its amino acid composition was rich in aspartic acid, glutamic acid, glycine and serine. This proteoglycan contained glycosaminoglycan chains that were composed mostly of chondroitin sulfate, with an average Mr=70,000 (SDS-PAGE) and Kav=0.44 (Sepharose CL-6B). The chemical composition of this proteoglycan from the rabbit alveolar bone was similar to that of the small proteoglycan from a mature bovine bone, whereas its size was a little bigger.
Practice　： Dentistry
Keywords　： Alveolar Bone, Proteoglycan, Rabbit