アブストラクト（14巻1/2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： A Detection Method of Acidogenic Bacteria
Subtitle　： ORIGINAL ARTICLES
Authors　： Koichi Kato*, Sakiko Yoshitake*, Mariko Aihara**, Hiroshi Kimoto**
Authors（kana）　：
Organization　： *Department of Biology, Nihon Women's Junior College of Hygiene, Kanagawa Dental College, **Department of Hygiene, Nihon Women's Junior College of Hygiene, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 14
Number　： 1/2
Page　： 45-56
Year/Month　： 1986 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Acidogenic bacteria were detected from oral cavities by using a reaction of the precipitate dissolution. The precipitates were made by the reaction with sodium-alginate and calcium chloride solution in a medium (mixing sodium-alginate and calcium chloride, the medium is turbid and develops precipitates; the pH range of this medium is nearly neutral). When oral streptococci (Stre. mutans, Stre. mitis and Stre. salivarius) were cultivated in the liquid medium containing the precipitates and sugars (sucrose and dextrose), it was found that the precipitates of calcium alginate dissolved in the bacterial acid produced from the fermentation of the sugars and the medium converted to gelatinous solid. When this reaction was attempted in/on the solid medium, the precipitates also dissolved around the growth colonies by the acid from the colony and developed a clear ring (zone). The size of these zones showed mostly acidogenic activity of the bacteria. Then applying, through this method, the isolation of acidogenic bacteria from an oral origin sample, the acid-producing bacteria were easily isolated from the specimen by the detection of the zone that the colonies made after cultivation.
Practice　： Dentistry
Keywords　： Acidogenic Bacteria, Calcium Alginate, Precipitate Dissolution, Streptococcus mutans