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アブストラクト(14巻1/2号:The Bulletin of Kanagawa Dental College)
English
Title : | An Application of Modified Tannin-Ferrocyanide・OsO4 Method for Bone Remodeling Study |
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Subtitle : | Short Communication |
Authors : | Seigyo So, Tsuneo Takahashi, Kazuto Takahashi |
Authors(kana) : | |
Organization : | Department of Oral Anatomy, Kanagawa Dental College |
Journal : | The Bulletin of Kanagawa Dental College |
Volume : | 14 |
Number : | 1/2 |
Page : | 63-65 |
Year/Month : | 1986 / 3 |
Article : | Report |
Publisher : | Kanagawa Odontological Society |
Abstract : | [Introduction] The mechanism of bone remodeling has been researched by a great number of investigators, but the large body of knowledge accumulated hitherto has not reduced the complexity but rather increased it. Most of the electron microscopic studies, both in vitro and in vivo, have also been limited to all the cells found along the bone surface without elucidating the relationships between the sequence of events and the surrounding vascularization. No mention has ever been reported about the distinction between the cells during bone remodeling. This study described how a modified tannin-ferrocyanide・OsO4 method can be used to distinguish the cells found at the bone remodeling site. [Materials ane Methods] Fragments of calvaria were taken from late-embryonic and newborn ICR strain mice and immediately immersed in 4% glutaraldehyde buffered with 0.1 M cacodylate (pH 7.3) for overnight, followed by rinsing with a 0.08 M cacodylate buffer at pH 7.3 containing 0.18 M sucrose, then further fixed without decalcification as follows: a) 1.5% potassium ferrocyanide and 1% tannic acid at pH 7.6-8.0 for 90 min at room temperature in the dark. b) Post-fixed in 0.1 M cacodylate-buffered 1% OsO4 for 90 min in the dark (pH 7.3). |
Practice : | Dentistry |
Keywords : | Mononuclear Phagocyte-Osteoclast Precursor, Preosteoblast, Osteoblast, Osteoclast, Modified Tannin-Ferrocyanide・OsO4 Method |