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アブストラクト(15巻1号:The Bulletin of Kanagawa Dental College)
English
Title : | Extraction of Lipopolysaccharides from Periodontopathic Bacteria |
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Subtitle : | ORIGINAL ARTICLES |
Authors : | Kenichi Sugizaki, Kiyoshi Imai, Moritaka Kimura, Kouichi Tanikawa, Hiroaki Inoue, Toshio Kawase, Katsuji Okuda*, Ichiro Takazoe*, Shigeru Saito |
Authors(kana) : | |
Organization : | Department of Oral Biochemistry, Kanagawa Dental College, *Department of Microbiology, Tokyo Dental College |
Journal : | The Bulletin of Kanagawa Dental College |
Volume : | 15 |
Number : | 1 |
Page : | 37-42 |
Year/Month : | 1987 / 3 |
Article : | Original article |
Publisher : | Kanagawa Odontological Society |
Abstract : | [Abstract] Lipopolysaccharides (LPSs) were extracted from the following periodontopathic bacteria; 1) Bacteriodes gingivalis (381), 2) B.intermedius (20-3), 3) B.melaninogenicus (ATCC 15930), 4) Actinobacillus actinomycetemcomitans (Y-4), 5) Capnocytophaga species (M-12), 6) Eikenella corrodens (1073) and 7) Fusobacterium nucleatum (ATCC 25586). LPSs were extracted by the hot phenol-water method as a water soluble material, and recovered with 0.70-4.43% of the bacterial dry weight. LPSs from Bacteroides has a lower pyrogenic activity than other genera and C.species LPS had the lowest activities. Constituents of each LPS were analyzed after a reduction with SDS-polyacrylamide gel electrophoresis. The electrophoretic pattern suggested that the LPS was constructed with some subunits in some rule. |
Practice : | Dentistry |
Keywords : | Lipopolysaccharide, Periodontopathic Bacteria, Pyrodic Test, SDS-Polyacrylamide Gel Electrophoresis |