アブストラクト(15巻1号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Extraction of Lipopolysaccharides from Periodontopathic Bacteria
Subtitle : ORIGINAL ARTICLES
Authors : Kenichi Sugizaki, Kiyoshi Imai, Moritaka Kimura, Kouichi Tanikawa, Hiroaki Inoue, Toshio Kawase, Katsuji Okuda*, Ichiro Takazoe*, Shigeru Saito
Authors(kana) :
Organization : Department of Oral Biochemistry, Kanagawa Dental College, *Department of Microbiology, Tokyo Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 15
Number : 1
Page : 37-42
Year/Month : 1987 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] Lipopolysaccharides (LPSs) were extracted from the following periodontopathic bacteria; 1) Bacteriodes gingivalis (381), 2) B.intermedius (20-3), 3) B.melaninogenicus (ATCC 15930), 4) Actinobacillus actinomycetemcomitans (Y-4), 5) Capnocytophaga species (M-12), 6) Eikenella corrodens (1073) and 7) Fusobacterium nucleatum (ATCC 25586). LPSs were extracted by the hot phenol-water method as a water soluble material, and recovered with 0.70-4.43% of the bacterial dry weight. LPSs from Bacteroides has a lower pyrogenic activity than other genera and C.species LPS had the lowest activities. Constituents of each LPS were analyzed after a reduction with SDS-polyacrylamide gel electrophoresis. The electrophoretic pattern suggested that the LPS was constructed with some subunits in some rule.
Practice : Dentistry
Keywords : Lipopolysaccharide, Periodontopathic Bacteria, Pyrodic Test, SDS-Polyacrylamide Gel Electrophoresis