アブストラクト(15巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Estimation of Acid Value Produced from Oral Streptococcal Colonies
Subtitle : SHORT COMMUNICATION
Authors : Koichi Kato*, Masako Nakamukai, Naoko Ishida, Hiromi Numata, Mariko Aihara, Hiroshi Kimoto
Authors(kana) :
Organization : *Department of Biology, Kanagawa Dental College, Hygiene, Nihon Women's Junior College of Hygiene, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 15
Number : 2
Page : 139-141
Year/Month : 1987 / 9
Article : Report
Publisher : Kanagawa Odontological Society
Abstract : [Introduction] The authors found a reaction in previous experiments that acids from bacteria dissolve the precipitate developed in the medium, cultivating acidogenic bacteria (Stre. mutans, Stre. salivarius, Stre. mitis) on/in the medium containing tryptic soy broth, agar, CaCl2 and Na-alginate. Then they isolated the acidogenic bacteria from the oral samples by using the precipitate as an indicator to detect the bacteria, and obtained the expected results. In this paper how organic acids affect the precipitate in the solid medium was tested and from the result it estimated the acid value produced from the oral streptococcal colonies. [Experimental materials and methods] The solid medium [containing finally 0.1% CaCl2, 1.0% dextrose, 1.7% tryptone, 0.3% soytone peptone, 0.25% dipotassium phosphate, 0.5% NaCl, 1.0% agar, 1.0% sodium alginate (one medium does not contain this reagent)], pH 7.0. The medium was sterilized at 121℃ for 15 minutes in an autoclave. A CaCl2 solution was stirringly mixed after the sterilization into the medium and poured into petri dishes (approximately 5mm thick) and a well of about 3mm in diameter was made on the medium.
Practice : Dentistry
Keywords : Acid Production, Acid Dissolution, Acidogenic Bacteria, Stre. mutans