アブストラクト(15巻2号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Solitary Cilia in Cultured Osteoblastic Cells
Subtitle : SHORT COMMUNICATION
Authors : Tsuneo Takahashi, Kei Matsumoto, Naohiro Shimozato, Kazuto Takahashi, Noriyoshi Kurihara*, Masayoshi Kumegawa**
Authors(kana) :
Organization : Department of Oral Anatomy, Kanagawa Dental College, *Department of Periodontology, Josai Dental University, **Department of Oral Anatomy, Josai Dental University
Journal : The Bulletin of Kanagawa Dental College
Volume : 15
Number : 2
Page : 143-146
Year/Month : 1987 / 9
Article : Report
Publisher : Kanagawa Odontological Society
Abstract : [Introduction] There have been many reports dealing with the presence of solitary cilia found in cells of hard tissues, such as in chondrocytes (Scherft and Daems 1967, Wilsman 1978), odontoblast (Garant 1967) and osteocyte and osteoblast (Tonna and Lampen 1972, Federman and Nichols 1974, Stanka 1975). However, there have been only a few reports of cilia in osteoblasts associated with organ culture. The present study deals with the solitary cilia (36 cases) which were accidentally found in osteoblastic cells of tissue culture of fetal mice calvarial bones. [Materials and Methods] The fetuses of pregnant mice of the ICR strain were removed on 19 and 20 days of gestation. Under a dissection microscope, their parietal bone fragments were dissected free of muscle and soft tissue, and cultured for up to 10 days in α-MEM supplemented with 10% fetal calf serum. The culturing was carried out in an atmosphere of 95% and 5% CO2. The cultured bone fragments were fixed in situ for 2 hours at 4℃ in 2% glutaraldehyde in a 0.1M sodium cacodylate buffer (pH 7.3). After washing overnight at 4℃ with a buffer containing 0.18M sucrose, the specimens were postfixed for 2 hours at 4℃ in 1% osmium tetroxide in the 0.1M sodium cacodylate buffer (pH 7.4) either with or without 0.1% ruthenium red (RR), stained en bloc for 20 minutes with a 2% uranyl acetate aquaous solution.
Practice : Dentistry
Keywords : Cilia, osteoblast, culture, ultrastructure