アブストラクト（16巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： The 60K Glycoprotein in Rat Calvarial Bone
Subtitle　： ORIGINAL ARTICLE
Authors　： Takao Kubota, Sadao Sato, Mitsuyoshi Kubota, Nobutaka Endo, Yoshii Suzuki, Toshio Kawase*
Authors（kana）　：
Organization　： Department of Orthodontics, Kanagawa Dental College, *Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 16
Number　： 1
Page　： 37-44
Year/Month　： 1988 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Noncollagenous proteins of the rat pup calvarial bone were isolated. One of them, an Mr 60,000 glycoprotein, was purified and characterized. The rat calvarial bone was extracted with 4M guanidium chloride (GdmC1) and subsequently demineralized with 0.5M EDTA + GdmC1. Both extracts contained protease inhibitors. Chromatography of the EDTA extracts on Sepharose CL-6B in 4M GdmC1, DEAE-Sephacel in 4M urea, and then rechromatography on Sepharose CL-6B resulted in purification of a glycoprotein with an apparent Mr 60,000. An amino acid analysis showed this protein to be rich in Asp and Glu. This protein did not cross-react with antibodies raised against rat serum protein. Osteoblast-like cells of the rat calvarial bone were cultured for study of the synthesis of the 60K glycoprotein. The cells were labeled for 24 h with 32Pi at the growth phase and the proteins in the medium were examined after SDS-PAGE. The results of autoradiography showed that 1,25(OH)2 vitamin D3 induced the synthesis of 60K glycoprotein. It is concluded that the 60K glycoprotein is a bone derived, vitamin D3 dependent protein.
Practice　： Dentistry
Keywords　： Noncollagenous Protein, Calvarial Bone, Phosphoprotein, 1,25(OH)2 Vitamin D3, 60K Glycoprotein