アブストラクト（17巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： The Study on High Molecular Weight Androgen Binding Protein in Submandibular Gland of Rat
Subtitle　： ORIGINAL ARTICLE
Authors　： Mayumi Tarui
Authors（kana）　：
Organization　： Department of Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 17
Number　： 2
Page　： 113-123
Year/Month　： 1989 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Testosterone (T) and 5α-dihydrotestosterone (DHT) binding to cytosol and nuclear extract were investigated in the submandibular gland (SMG) and ventral prostate (VP) of rat at 3 and 7 weeks of age. The concentration of T and DHT were also measured by a specific radioimmunoassay (RIA) in the cytosol and nuclei of the tissues. The androgen binding protein analyzed by Sephacryl S-200 column chromatography showed a single binding peak in the nuclei and two critical peaks in the cytosol at 7 weeks old SMG and VP. The binding proteins were recovered predominantly as the mol wt of 150-200kDa and 14-20kDa in the cytosol and as a mol wt of 14-20kDa in the nuclei. The two kinds of androgen binding protein were also revealed in the sytosol of 3 weeks old rat SMG. However, the S-200 column chromatography did not reveal the 150-200kDa protein in the prostatic cytosol from 3 weeks rat, showing one binding peak of 14-20kDa protein. The 150-200kDa protein was specific to T in SMG at 3rd week and to DHT at 7th week in VP. The amount of specific binding of DHT to the 150-200kDa protein was the greatest in VP cytosol of 7th week. The specific binding to the nuclear protein was shown by T in SMG at 3rd week and by DHT in VP at 7th week. High androgen concentrations were observed in the nuclei of male SMG (T : 3.92 +- 0.72ng/g, DHT : 4.14 +- 0.51ng/g) and the values were higher than in the nuclei of VP (T : 2.69 +- 0.70ng/g, DHT : 2.85 +- 0.67ng/g) at 3 weeks old. The values in SMG decreased to the levels of 1.67 +- 0.68ng/g for T and 1.19 +- 0.09ng/g for DHT at 7 weeks. The accumulation of T and DHT in the nuclei of VP were shown at 7 weeks old, revealing 4.29 +- 3.18ng/g for T and 5.53 +- 2.39ng/g for DHT. The cytosolic androgen levels in VP rather decreased from the 3rd week (T : 2.78 +- 1.20ng/g, DHT : 3.57 +- 2.31ng/g) to the 7th week (T : 1.33 +- 0.40ng/g, DHT : 1.80 +- 0.66ng/g). The T and DHT values in male cytosol and female cytosol and nuclei of SMG did not show a distinct increase or decrease with age. Thus the SMG androgen dependency is characterized by the existence of high molecular weight (150-200kDa) protein bound to androgen in cytosol at 3 weeks age, and high concentration of T and DHT in the nuclei at the stage. These facts imply that rat SMG is affected by androgen at earlier stage than VP and that the definite androgen dependency is decided by 3 weeks old.
Practice　： Dentistry
Keywords　： Submandibular Gland, Androgen, High Molecular Weight Binding Protein