アブストラクト（19巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Activation of Ovarian Aromatase by Unidentified Factor in Rat Submandibular Gland
Subtitle　： ORIGINAL ARTICLES
Authors　： Teruo Yoneda, Mayumi Tarui, Shinri Koshika
Authors（kana）　：
Organization　： Department of Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 19
Number　： 2
Page　： 77-83
Year/Month　： 1991 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] To investigate the physiological role of the submandibular gland (SMG) on the female reproductive systems, the aromatase activities were assayed in the ovarian microsomal fraction obtained from sialoadenectomized rats. The microsome was incubated with 3H-Δ4-androstenedione (Δ4-dione) in the presence of NADPH. Sialoadenectomy (surgical removal of the submandibular gland) markedly reduced the enzyme activities in the ovary of immature rats. The enzyme kinetics showed the reduced Vmax of the enzyme for the substrate Δ4-dione, control rat, 8.3pmol 3H2O/mg protein per hour; in treated rat, 2.1pmol, however, the Km value (0.03μM) showed neither an increase nor decrease by the ectomy. When Δ4-dione and ovarian microsomes were incubated in the presence of the extract of the SMG, the reduced Vmax was restored to more than the normal level. NGF, EGF, FSH, LH and insulin were not affected on the enzyme activities in this in vitro study. The plasma concentration of LH was significantly reduced by the ectomy. From these results obtained, it is concluded that the rat SMG contains an unidentified factor which activates the ovarian aromatase activity, and it is possible that the factor acts on the pituitary-ovary system.
Practice　： Dentistry
Keywords　： Aromatase, Ovary, Sialoadenectomy