アブストラクト（20巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Effect of Cyclic Mechanical Stress on Proliferation and Differentiation of Human Periodontal Ligament Fibroblasts
Subtitle　： ORIGINAL ARTICLES
Authors　： Nobuhiro Yasu
Authors（kana）　：
Organization　： Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 20
Number　： 1
Page　： 22-29
Year/Month　： 1992 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The culture of human periodontal ligament fibroblasts (HPLF) was allowed to be subjected to mechanical stress for growth and confluent periods of cell culture. HPLF responded to cyclic mechanical stress, as evidenced by DNA synthesis and alkaline phosphatase (ALPase) activity. Cells were cultured with a continuous deformation comprised of 3 cycles per min of 6%, 18%, and 24% elongation, i.e., magnitude of stress. The rate of DNA synthesis decreased down to 0.71-fold during the growth phase and increased up to 1.28-fold during the confluent phase at 2 days in 24% stress compared with the non-stress culture. Further, stressed HPLF in culture increased ALPase activity during the growth phase in 24% stress (e.g., 138% of control at 2 days after incubation). However, during the confluent phase in 18% stress at 2 days, ALPase activity showed a 26% decrease. In addition to the effects of mechanical stress, the conditioned medium of stress regimen was also explored with regard to ALPase activity. The result of a 37% increase on ALPase activity was obtained with HPLF cultured in the conditioned medium. These findings suggest that mechanical stress may regulate the function of proliferation and differentiation of HPLF during the growth and confluent phase in vitro.
Practice　： Dentistry
Keywords　： Human Periodontal Ligament Fibroblasts, Mechanical Stress, Alkaline Phosphatase, Proliferation, Differentiation