アブストラクト(20巻1号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Effect of Cyclic Mechanical Stress on Attachment and Spreading of Human Periodontal Ligament Fibroblasts
Subtitle : ORIGINAL ARTICLES
Authors : Nobuhiro Yasu, Taisuke Kawamura, Yoshio Kakai, Shinji Deguchi*, Yoshio Kato, Takafumi Sakamoto, Toshio Kawase, Shigeru Saito
Authors(kana) :
Organization : Departments of Oral Biochemistry, Kanagawa Dental College, *Departments of Periodontology, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 20
Number : 1
Page : 30-37
Year/Month : 1992 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] The growth of normal fibroblasts is in need of an adhesive substratum on which to spread. An attachment activity was measured because it has been shown to be required for growth and spreading of human periodontal ligament fibroblasts (HPLF). The degree of spreading was controlled by coating the conditioned medium in hydrophobic culture plates. This medium was derived from HPLF exposed to varying magnitudes of mechanical stress, comprised of 3 cycles per minute of 0, 6, 18 and 24% elongation. HPLF immediately transformed and spread in the culture plate by coating the conditioned medium in the stress culture compared with the blank. Surprisingly, HPLF were rapidly transformed and spread by the conditioned medium of 24% stress more than that of 6% stress. The present data suggest that the requirement for spreading may in part by mediated be mechanical stress.
Practice : Dentistry
Keywords : Human Periodontal Ligament Fibroblasts, Mechanical Stress, Conditioned Medium