アブストラクト(21巻1号:The Bulletin of Kanagawa Dental College)

The Bulletin of Kanagawa Dental College

English

Title : Partial Purification of Cell Attachment and Spreading Factors Derived from the Conditioned Medium of Human Periodontal Ligament Fibroblasts
Subtitle : ORIGINAL ARTICLES
Authors : Takafumi Sakamoto, Katsuhiro Nishiyama, Toshio Kawase
Authors(kana) :
Organization : Department of Oral Biochemistry, Kanagawa Dental College
Journal : The Bulletin of Kanagawa Dental College
Volume : 21
Number : 1
Page : 25-34
Year/Month : 1993 / 3
Article : Original article
Publisher : Kanagawa Odontological Society
Abstract : [Abstract] The primary object of this research was to study the mechanism of regeneration of periodontium in the treatment of periodontal diseases. Recently our studies have demonstrated that the conditioned medium (HPLF-CM) of human periodontal ligament fibroblasts (HPLF) contains the cell attachment and spreading factors (CASFs), which are different types of fibronectin (FN) and vitronectin (VN). The purpose of this study was to evaluate the procedure for preparation and purification of CASFs using an affinity chromatography. After a confluent phase of cultured HPLF, HPLF-CM derived from HPLF cultured with serum-free MCDB 107 medium for 24 hr with or without 10 μg/ml cycloheximide was harvested, centrifuged and condensed by the method of ultrafiltration (YM-5 membrane). The HPLF-CM was applied to the affinity chromatography of cellulofine bound with IgG of polyclonal antibody against the whole bovine serum. The noninteracted fraction of affinity chromatography of HPLF-CM had the CASF activity. After this fraction was applied to IEC-DEAE HPLC, the CASF active fractions eluted with a wide range of ion concentration from 0.37 to 0.41 M NaCl were obtained. As the CASF activity of HPLF-CM was inhibited by cycloheximide, the CASFs released by HPLF could be synthesized by the de novo system. There were several molecules without FCS derived components which demonstrated CASF activity. Therefore, the processes of cell attachment and spreading factors may be correlatively regulated by the CASFs.
Practice : Dentistry
Keywords : Human Periodontal Ligament Fibroblasts, Cell Attachment and Spreading Factors, FMP Activated Cellulofine