アブストラクト（22巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Study on the Abnormality of Tumor Suppressor Genes p53 and RB in Human Oral Cancer Cell Lines
Subtitle　： ORIGINAL ARTICLES
Authors　： Yoshiko Iwasaki, Yoshinobu Kubota*, Shinri Koshika
Authors（kana）　：
Organization　： Department of Biochemistry, Kanagawa Dental College, *Department of Urology,Yokohama City University
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 22
Number　： 2
Page　： 91-100
Year/Month　： 1994 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The abnormality of the tumor suppressor gene, p53 and the RB genes, was studied in human cancer cell lines derived from cancer of oral cavity and associated cancer, using the methods of single strand conformation polymorphism (SSCP) analysis of RNA and polymerase chain reaction (PCR)-quantitation. We found the structural alteration of the p53 gene and RB gene in KB (squamous cell carcinoma of oral cavity) cells and HMV-1 (malignant melanoma) cells, and the absence of RB gene expression in HMV-1 cells from the results of PCR-quantitation. These results suggest that the structural alteration and the abnormal expression of p53 and RB genes could be involved in the development of cancers of oral cavity. The importance of the validation to analyzing the tumor supressor genes in clinical samples of human oral cancers are also indicated.
Practice　： Dentistry
Keywords　： Tumor Suppressor Genes-p53 gene, RB gene-RNA-SSCP Method-Polymerase Chain Reaction