アブストラクト（23巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Cell Attachment and Spreading Factors of Bovine Dentin Extract
Subtitle　： ORIGINAL ARTICLES
Authors　： Masahiro Saito
Authors（kana）　：
Organization　： Department of Restorative Dentistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 23
Number　： 1
Page　： 9-16
Year/Month　： 1995 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The interactions between root surface and periodontal connective tissue are not completely understood. It is also unclear whether molecules influence periodontal connective tissue formation and regeneration. Kubo and Kawase reported that human periodontal ligament fibroblasts (HPLF) at a confluent phase synthesize the cell attachment and spreading factors (CASFs) in the conditioned medium (HPLF-CM). CASFs might be important in the regeneration mechanisms which direct the behavior of cells, such as cell attachment, proliferation and differentiation. In the case of periodontal disease, we must completely remove the diseased cementum. However if root dentin includes CASFs of HPLF, the dentin may affect the regeneration of periodontal connective tissue. This study examines the effect of dentin components on the attachment and spreading of periodontal ligament fibroblasts. Dentin was harvested from bovine tooth and extracted by sequential procedures using 4 M guanidine HCI (GI-ext), 0.5 M EDTA (E-ext) and 4 M guanidine HCI (G2-ext). The cell attachment and spreading assay was measured using HPLF on tissue culture plates previously incubated with each dentin extract. The results showed that GI-ext mediated high CASF activity compared with that of G2-ext. E-ext showed no activity. The CASF activity of GI-ext appeared to be almost identical to HPLF-CM. Immunoblot analysis demonstrated that antibodies of type I collagen and 55 kDa cementum-derived attachment protein (CAP) cross-reacted with GI-ext but antibodies of fibronectin and vitronectin did not cross-react. With gel filtration and HPLC in an IEC-DEAE column, four fractions with CASF activity were fractionated in GI-ext. It is concluded that dentin contains CASFs of HPLF and that these substances may play an important role in periodontal regeneration by facilitating HPLF attachment and spreading at the root dentin surface.
Practice　： Dentistry
Keywords　： Cell attachment and spreading factors, Dentin, Human periodontal ligament fibroblast, Cementum