アブストラクト（23巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Neutrophil-Mediated Damage to Human Periodontal Ligament Derived Fibroblasts
Subtitle　： BKDC CLINICAL AND RESEARCH TOPICS INFECTION
Authors　： Shinji Deguchi1, Toshio Hori1, Toshio Kawase2, Shigeru Saito2
Authors（kana）　：
Organization　： 1Department of Periodontology, Kanagawa Dental College, 2Department of Oral Biochemistry, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 23
Number　： 2
Page　： 141-144
Year/Month　： 1995 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] It was demonstrated that oxygen radicals and neutral protease released from Polymorphonuclear leukocytes (PMNs) were toxic to fibroblast. We recently showed that lipopolysaccharide (LPS) enhanced N-formyl-methionyl-leucyl-phenylalanine (FMLP)-stimulated PMNs mediated damage to cultured human periodontal ligament fibroblast (HPLF). The mechanism by which these oxygen radicals and the release of tissue reactive proteins cause damage to HPLF is unknown. Superoxide (O2-), H2O2, -OH, 1O2 have all been implicated as the toxic agents when cells are exposed to oxygen radicals. It is important to determine which oxygen radicals are responsible for cellular damage in order to elucidate the mechanism of cytotoxicity. The present study was designed to evaluate the effect of these oxygen radicals for PMNs-mediated damage to HPLF. Fibroblast was cultured to a confluent stage and was subjected to LPS (1000 ng/ml) and FMLP (10-6M) stimulated PMNs, and then exposed to scavengers of various oxygen radicals simultaneously. Superoxide dismutase (SOD) (10 μg/ml ; 3,300 U/mg) with or without catalase (100 μg/ml ; 6,900 U/mg) had no significant inhibitory effect on PMNs mediated fibroblast injury during the three hour assay. These results suggest that, in this system, FMLP and LPS-stimulated PMNs-mediated damage to the fibroblast may not be mediated by O2-H2O2. This damage may be mediated in large part by other oxygen radicals and the neutral protease.
Practice　： Dentistry
Keywords　： Periodontium, Infection, Neutrophils, Cytotoxicity, Oxidants