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アブストラクト(24巻1号:The Bulletin of Kanagawa Dental College)
English
Title : | Expression of Trypsinogen mRNA in Squamous Cell Carcinoma Cell Line from 7, 12-Dimethylbenz[a]anthracene-Induced Rat Submandibular Gland |
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Subtitle : | SHORT COMMUNICATION |
Authors : | Yasumasa Kato, Satoru Ozono1, Shinri Koshika |
Authors(kana) : | |
Organization : | Department of Oral Biochemistry, Kanagawa Dental College, 1Department of Oral Pathology, Kanagawa Dental College |
Journal : | The Bulletin of Kanagawa Dental College |
Volume : | 24 |
Number : | 1 |
Page : | 15-18 |
Year/Month : | 1996 / 3 |
Article : | Report |
Publisher : | Kanagawa Odontological Society |
Abstract : | [Abstract] A squamous cell carcinoma cell line (RSS18) derived from a 7, 12-dimethylbenz[a]anthracene-induced submandibular gland of a femal rat was tested for production of ECM-degradative proteinases. Zymographic analysis showed that gelatinolytic activities associated with 85-and 25-kDa proteins were secreted in a serum-free medium. Enzyme-inhibition assays were performed to determine the proteinase type. The gelatinolytic activities of the 85-and 25-kDa proteins were completely inhibited by diisopropyl fluorophosphate, suggesting that they belong to the serine proteinase type. When the conditioned medium was treated with enteropeptidase at 37℃ for 10min before zymographic analysis, the gelatinolytic activity of the 25-kDa protein significantly increased. Moreover, reverse transcription-polymerase chain reaction analysis revealed that trypsinogen mRNA was expressed in the cells. These results suggested that 85- and 25-kDa proteins were similar to plasmin and trypsin proteinases, respectively. |
Practice : | Dentistry |
Keywords : | Trypsinogen, Squamous cell carcinoma, Submandibular gland, DMBA |