アブストラクト（24巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Fluorescent Three-dimensional Views Cell Nuclear Analysis of Apoptosis
Subtitle　： BKDC CLINICAL AND RESEARCH TOPICS : Apoptosis
Authors　： Keiichi Tsukinoki, Yoshiko Iwasaki, Yasushi Yamazaki, Shigeo Komatsu, Yuzo Kamoi, Yoshihisa Watanabe
Authors（kana）　：
Organization　： Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 24
Number　： 2
Page　： 91-96
Year/Month　： 1996 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Apoptosis is defined primarily based on the morphological characteristics of cells, and can be determined only through morphological observation. Therefore, morphologically multifarious observations provide the most important basis for judgement. A confocal laser scanning microscope is superior to any conventional microscope in definition and resolving power, especially along the Z-axis. By means of ACAS570 (confocal laser scanning microscope), the distribution of nuclear DNA content and the three-dimensional analysis were very useful in the detection of morphological apoptosis. In this report, for the purpose of clarifying morphological changes in nuclei of apoptosis, a thorough description of the new analytical methods using a confocal laser scanning microscope are given.
Practice　： Dentistry
Keywords　： Apoptosis, Confocal laser scanning microscope (CLSM), Karyomorphism, Simulated fluorescence process (SFP), ACAS570