アブストラクト（24巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Study on Apoptosis in the Developing Rat Submandibular Gland
Subtitle　： BKDC CLINICAL AND RESEARCH TOPICS : Apoptosis
Authors　： Hiroyuki Hayashi
Authors（kana）　：
Organization　： Department of Oral Histology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 24
Number　： 2
Page　： 111-114
Year/Month　： 1996 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The distal portion of the prepubertal rat submandibular gland consists of terminal tubules. In this portion, three types of cells were observed : acinar cells (A-cells), proacinar cells (PA-cells), and terminal tubule cells (TT-cells). An intercalated duct and a striated duct appeared continuously with the terminal tubules. Electron microscopy showed the absence of PA-cells from 14 days after birth. Paraffin sections stained with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method showed negative reactions in all cells until 14 days after birth. The TUNEL method showed positive reactions in a small number of cells in the TT-cells. The number of positive cells increased until 28 days after birth. Electron microscopy revealed nuclear chromatin aggregation, suggesting apoptosis in TT-cells 21 days or more after birth. Phagocytosis of the apoptotic body by intercalated duct cells was observed. These results suggest the close involvement of apoptosis in the regulation of cells constituting the submandibular gland in the postnatal growth of the rat submandibular gland.
Practice　： Dentistry
Keywords　： Apoptosis, Development, Submandibular gland, TUNEL method