アブストラクト（25巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： The Role of Integrin β1 during Mineralization of the Tooth Germ
Subtitle　： BKDC CLINICAL AND RESEARCH TOPICS : Adhesion Molecule
Authors　： Mineyo Iwase
Authors（kana）　：
Organization　： Department of Oral Histology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 25
Number　： 2
Page　： 89-92
Year/Month　： 1997 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Integrin receptors for extracellular matrix (ECM) molecules have been thought to play important roles in organ morphogenesis since they mediate embryonic cells adhesion and their migration. By the use of a immunohistochemical technique, the localization of integrin β1 and focal adhesion-associated protein tyrosine kinase (FAK) were examined in murine tooth germs. The immunoreactivity of integrin β1 was found to increase in the distal ends of the inner dental epithelium accompanied by the accumulation of fibronectin. FAK was expressed at unique differentiation stages in both odontoblast and ameloblast lineage. During this stage, odontoblasts and ameloblasts changed in shape and predentin formation started. Furthermore, integrin β1 translation arrest was accomplished by the antisense phosphorothioate oligodeoxynucleotide (ODN). All of the control explants cultured showed a normal structure in the formation of enamel and dentin, as well as tooth germ observed in vivo. In contrast, the antisense-treated explants did not show the formation of enamel and dentin, although they showed the normal crown morphogenesis of teeth. These results suggest that the integrin β1 may take part in the mineralization process of the developing tooth.
Practice　： Dentistry
Keywords　： Integrin β1, Tooth development, Mineralization, Antisense oligodeoxynucleotide, Cytoskeleton