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アブストラクト（27巻1号：The Bulletin of Kanagawa Dental College）
English
| Title ： | Transcriptional regulation of bone sialoprotein gene in differentiated osteoblast cell line derived from normal mouse bone marrow and its involvement of Cbfa1/Pebp2αA1 |
|---|---|
| Subtitle ： | Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998 |
| Authors ： | Masato Yamauchi, Kenichi Sasaguri, Eiji Hasegawa, Sadao Sato, Akihiro Umezawa1) |
| Authors（kana） ： | |
| Organization ： | Department of Orthodontics, Kanagawa Dental College, 1)Department of Pathology, Keio University of School of Medicine |
| Journal ： | The Bulletin of Kanagawa Dental College |
| Volume ： | 27 |
| Number ： | 1 |
| Page ： | 61-63 |
| Year/Month ： | 1999 / 3 |
| Article ： | Report |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | [ABSTRACT] To elucidate the mechanisms of tissue specific gene expression of BSP, we have cloned 16kbp from 5' flanking region of mouse BSP gene and investigated the functional relevance between Cbfa1/Pebp2∂A1 and cis-acting elements. 5' deletion analyses of BSP promoter-luciferase chimeric constructs showed that the promoter activity of p4.5kb-Luc encompassing both distal and proximal Cbfa1/Pebp2∂A1 site was enhanced ～ 6 fold on cotransfection with the Cbfa1/Pebp2∂A1 expression vector whereas that of p4.2kbp-Luc encompassing proximal site was not in KUSA-A1 (differentiated osteoblasts with expressing BSP). The enhancement of distal Cbfa1/Pebp2∂A1 site was completely ablogated in KUSA-O (nondifferentiated osteoblasts without expressing BSP). Western blot analyses using mouse anti-mouse Pebp2∂A1 monoclonal antibody showed that both KUSA-A1 and KUSA-O synthesized the relative large amount of Cbfa1/Pebp2∂A1 protein. These results identified the functional Cbfa1/Pebp2∂A1 responsive element and suggested that Cbfa1/Pebp2∂A1 was requisite for the transcriptional regulation of BSP but another additional factor was participated in the differentiated osteoblast. |
| Practice ： | Dentistry |
| Keywords ： | Bone sialoprotein, Cbfa1/Pebp2∂A1, Gene transcription, Promoter |
