アブストラクト（27巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Quantity Analysis of Nitric Oxide (NO) by Iron Complex with Dithiocarboxy Sarcosine : Its Application to Determine the Direct Effect of NO on Cardiac Sarcoplasmic Reticulum Ca2+ -ATPase Activity
Subtitle　： Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998
Authors　： Hirofumi Shoji, Chang-il Lee, Eiichiro Okabe
Authors（kana）　：
Organization　： Department of Pharmacology and ESR Laboratory, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 27
Number　： 1
Page　： 70-72
Year/Month　： 1999 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] In the present study, we tested the nitric oxide (NO) trapping efficiency of dithiocarbamate derivative N-(dithiocarboxy) sarcosine (DTCS) and assessed the accuracy of NO measuring system utilized electron spin resonance spectroscopy. We also investigated the direct effect of NO on Ca2+ -ATPase activity of cardiac sarcolpasmic reticulum (SR). Iron complex with DTCS (Fe(DTCS)2) was used as spintrapping reagent for NO. Fe(DTCS)2 complex reacted with NO released from NO donor NOC-7 to give an NO-Fe(DTCS)2 complex at 37℃. The spin concentration of formed NO-Fe(DTCS)2 was linearly increased in a NOC-7 concentration-dependent manner (r=1.00); the lowest detecting limit was 1μM NO. In the series of experiments for confirming the direct effect of NO, 30min of incubation of SR with 1mM NOC-7 (released cumulative NO concentration was 0.25mM) produced significant reduction of Ca2+ -ATPase activity; sulfhydryl group (-SH) content of SR vesicles was also decreased. Hence NO can depress Ca2+ -ATPase activity via modification of the key -SH groups on the ATPase molecule of the SR.
Practice　： Dentistry
Keywords　： Nitric oxide, Electron spin resonance, ESR, Dithiocarbamate, Cardiac sarcoplasmic reticulum, Ca2+ -ATPase