アブストラクト（27巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Effects of reactive oxygen species on colonic AH/Type2 myenteric neurons
Subtitle　： Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998
Authors　： Satoko Wada-Takahashi, Noriyuki Yuyama, Kenji Tamura, Hirofumi Shoji*, Kazuo Todoki*, Eiichiro Okabe*
Authors（kana）　：
Organization　： Department of Oral Physiology, Kanagawa Dental College, *Department of Parmacology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 27
Number　： 1
Page　： 76-78
Year/Month　： 1999 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [ABSTRACT] With conventional intracellular recording methods, effects of reactive oxygen species (ROS) on the electrophysiological behaviors of AH/type2 myenteric neurons in the guinea pig distal colon were examined. Application of hypoxanthine (HX) and xanthine oxidase (XO) induced a transient membrane hyperpolarization, followed by a long-lasting membrane excitation in most of AH/Type2 neurons. HX-XO induced hyperpolarization and depolarization were not changed by addition of tetrodotoxin (TTX, 0.2μM), indicating direct actions of ROS on the postsynaptic membrane. Superoxide dismutase (SOD) and catalase partially reversed membrane hyperpolarization, while excitatory response was completely blocked by dimethyl sulfoxide (DMSO). These results indicated that ROS produced by HX-XO reaction have both excitatory and inhibitory actions on the colonic AH/Type2 neurons. ROS generated within the gut wall might be exerting a physiological role in the control of the gastrointestinal function by acting on the enteric neural circuits.
Practice　： Dentistry
Keywords　： Reactive oxygen species, Myenteric neurons, AH/Type2 neurons, Colon, Guinea pig