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アブストラクト(27巻1号:The Bulletin of Kanagawa Dental College)
English
Title : | Role of LFA-1 in the Osteoclast Formation |
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Subtitle : | Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998 |
Authors : | Nobuyuki Tani-Ishii, Akira Tsunoda, Gouichi Matsumoto1), Toshio Teranaka, Toshio Umemoto2) |
Authors(kana) : | |
Organization : | Department of Operative Dentistry and Endodontics Kanagawa Dental College, 1)Department of Oral surgery, Kanagawa Dental College, 2)Department of Oral Microbiology Kanagawa Dental College |
Journal : | The Bulletin of Kanagawa Dental College |
Volume : | 27 |
Number : | 1 |
Page : | 79-81 |
Year/Month : | 1999 / 3 |
Article : | Report |
Publisher : | Kanagawa Odontological Society |
Abstract : | [ABSTRACT] The leukocyte integrin LFA-1 (CD11a/CD18) plays an important role in lymphocyte recirculation and homotypic interactions. Recently, it has been reported that LFA-1 and ICAM-1 are involved in the formation of osteoclast. In this study, the role of LFA-1 in osteoclast formation via interaction between stromal cells and osteoclast progenitors and/or among osteoclast progenitors was investigated. Osteoclast formation was observed on coculture of LFA-1 deficiency mouse (CD11a-/-) bone marrow cells and MC3T3-G2/PA6 cells on a dentin slice in the presence of 1α,25(OH)2D3 and dexamethasone for 7 days. At the end of culture period, the number of TRAP-positive cells was counted and the resorption pits area were evaluated by reflected light microscopy. The number of TRAP-positive cells was significantly decreased (176.4+-11.7 vs 260.0+-20.1 (control: C57BL/6N) cells/1x106μm2) and the total pits area per dentin slice was also decreased (79,911+-1,075 vs 113,236+-9,818 (control: C57BL/6N)μm2/3x105μm2) in CD11a-/- mouse. On the other hand, the ratio of TRAP-positive mononuclear to multinuclear cells was not changed. These results show that adhesion molecules LFA-1 may play a role in osteoclast formation via interaction between stromal cells and osteoclast progenitors. |
Practice : | Dentistry |
Keywords : | LFA-1 deficiency, Osteoclast, MC3T3-G2/PA6 |