アブストラクト（27巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Characteristics of Interaction between Luminol Chemiluminescence and a Metal Component Enzyme
Subtitle　： Selective Proceedings of 33rd General Meeting of Kanagawa Odontological Society, 1998
Authors　： Kazuo Todoki, Eiichiro Okabe
Authors（kana）　：
Organization　： Department of Pharmacology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 27
Number　： 1
Page　： 85-87
Year/Month　： 1999 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] The present study was undertaken to determine the mechanism by which catalase or horseradish peroxidase (HRP) enhances luminol chemiluminescence (CL) in the presence of H2O2. The H2O2 (which concentration did not emit luminol CL by itself)/luminol CL was enhanced by FeSO4, HRP and catalase, in a concentration-dependent fashion. The enhanced luminol CL was diminished by iron chelator deferoxamine, suggesting that the observed effects afforded by HRP and catalase are the reaction via iron iondependent mechanism(s). The inhibitors of catalase and HRP had no effect on this reaction system. Hence, catalase and HRP may enhanced H2O2-induced luminol CL independently of its peroxidase activity.
Practice　： Dentistry
Keywords　： Luminol, Chemiluminescence, Catalase, Horseradish peroxidase, Deferoxamine, Deoxygenation