アブストラクト（29巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Expression of Fibroblast Growth Factor Receptor 3 During Tooth Germ Development
Subtitle　： ORIGINAL ARTICLES
Authors　： Keiichi Tsukinoki, Takayuki Yamamoto, Yoshiko Miyoshi, Yasuhumi Koike, YUZO Kamoi, Masamoto Murakami, Yoshihisa Watanabe
Authors（kana）　：
Organization　： Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 29
Number　： 1
Page　： 3-7
Year/Month　： 2001 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] In the present study, we investigated the distribution of fibroblast growth factor receptor 3 (FGFR3) during human tooth development as revealed by immunohistochemical methods, in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR). To the best of our knowledge, this is the first study that describes the expression of FGFR3 in human tooth germs. The appearance of FGFR3 protein and mRNA were examined in series sections of fetal jaws (13, 14 and 34 weeks of gestation) by streptavidin-biotin complex-HRP system and ISH utilizing T-T dimerized oligonucleotide probes. The weak expression of FGFR3 was consistently seen in the enamel organ except for the stellate reticulum. In the crown stage of tooth germ, FGFR3 protein and mRNA were strongly detected in ameloblasts and odontoblasts during hard tissue formation, but no expression in preameloblasts and preodontoblasts. FGFR3 mRNA signals were also seen in the tooth germ of the crown stage by RT-PCR. No expression of FGFR3 was revealed in dental papillae and dental follicles by the immunohistochemical methods and ISH. From the results of our study, it is suggested that expression of FGFR3 has a strong correlation with the development of the fetal tooth germ and FGFR3 might be playing an important role in the differentiation of ameloblast or odontoblast and in deposition of enamel or dentin matrix.
Practice　： Dentistry
Keywords　： Immunolocalization, Human tooth germ, Fibroblast growth factor receptor 3