アブストラクト（29巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Tumor Growth Inhibited by the Expression of Endostatin cDNA in Murine Squamous Cell Carcinoma
Subtitle　： ORIGINAL ARTICLES
Authors　： Yasushi Ohmi, Goichi Matsumoto, Junichi Shindo
Authors（kana）　：
Organization　： First Department of Oral and Maxillofacial Surgery, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 29
Number　： 2
Page　： 67-74
Year/Month　： 2001 / 9
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] To determine whether endostatin, a powerful angiogenesis inhibitor, is capable of suppressing the tumor growth of head and neck squamous cell carcinoma in a murine model, we transfected one or the other of endostatin cDNA and Neo into mouse squamous cell carcinoma NRS-1 and SCC-VII cells in vitro. We subcutaneously implanted 2.5 x 106 transfected tumor cells carrying either endostatin DNA or not into 6-week-old female C3H/He mice. Tumor volumes were measured until 4 weeks. An immunohistochemical study was performed using antibodies against CD31,vascular endothelial growth factor (VEGF), and fibroblast growth factor-2 (FGF-2). Apoptotic cells were assessed by TUNEL staining. Tumor growth was significantly inhibited when the tumor cells were transfected with endostatin. Inhibition of endostatin-transfectant tumors was accompanied by a marked reduction in vascularity and by the presence of many apoptotic tumor cells. Endostatin cDNA did not affect the expression of VEGF and FGF-2 in the vascular endothelium. In this study, we demonstrated the efficacy of endostatin gene transfer in suppression of both tumor growth and tumor vascularity in a murine model, suggesting its potential efficacy in the treatment of the corresponding carcinoma in humans.
Practice　： Dentistry
Keywords　： Endostatin, Gene therapy, Head and neck cancer