アブストラクト（29巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Isolation and characterization of Dental Follicle Cells derived from bovine developing tooth germ
Subtitle　： Selective Proceedings of 35th General Meeting of Kanagawa Odontological Society, 2000
Authors　： Keisuke Handa*1, Masahiro Saito*1,*2, Masato Yamauchi*3, Akira Tsunoda*1, Shintaro Hattori*4, Keiichi Tsukinoki*5, Sadao Sato*3, Minoru Toyoda*4, Yoshihisa Watanabe*5, Toshio Teranaka*1,*2
Authors（kana）　：
Organization　： *1Department of Operative Dentistry and Endodontics, Kanagawa Dental College, *2Institute of Oral Health Science, Kanagawa Dental College, *3Department of Orthodontics, Kanagawa Dental College, *4Department of Prosthodontics, Kanagawa Dental College, *5Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 29
Number　： 1
Page　： 28-30
Year/Month　： 2001 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Dental follicle is the fibrous tissue, which surrounds developing tooth germ, and it is believed to contain a variety of progenitors for periodontium such as cementoblasts, periodontal ligament cells and osteoblasts. To address the molecular mechanisms of cementogenesis, we have isolated and characterized the putative cementoblast progenitors from dental follicle. Dental follicle was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released cells were maintained as a bovine dental follicle cells (BDFC). To elucidate the differentiation capacity of BDFC, they were transplanted into the subcutaneous tissue of severe combined immunodeficiency (SCID) mice for 4 weeks. Transplanted BDFC formed cementum-like matrix and it was positive for anti cementum attachment protein monoclonal antibody. The BDFC transplants revealed the expression of mRNA for osteocalcin, osteopontin, type I collagen and bone sialoprotein. These results indicated that the BDFC contained cementoblast progenitors, and provide a useful model for investigating molecular mechanisms of cementogenesis.
Practice　： Dentistry
Keywords　： Cementogenesis, Dental follicle, Tooth germ, Progenitor, Differentiation