アブストラクト（29巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： In Vivo Electron Spin Resonance (ESR) Imaging : A Basic Research for Bioradical Detection System in Oral Tissues
Subtitle　： Selective Proceedings of 35th General Meeting of Kanagawa Odontological Society, 2000
Authors　： Hirofumi Shoji, Hiroyuki Miyazaki, Yoichi Omori, Chang-il Lee
Authors（kana）　：
Organization　： Department of Pharmacology, ESR Laboratory, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 29
Number　： 1
Page　： 34-35
Year/Month　： 2001 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] In the present study, we improved an in vivo ESR imaging system to detect free radicals and to obtain its exact distribution in biological model. Furthermore, the in vivo ESR-CT imaging system we improved was applied to the rat brain and mouse head region after treatment with two different nitroxide radical spin probes; the blood-brain barrier (BBB) permeable nitroxide radical, 3-methoxycarbonyl 2,2,5,5-tetramethylpirrolidine N-oxyl (MC-PROXYL), and BBB-impermeable-nitroxide radical, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (C-PROXYL) were treated. In the preliminary study, we measured phantom model including nitroxide spin probe. We obtained the shape image as well as phantom up to O.25 mM. In the experiment of isolated rat brain after injection of MC-PROXYL via femoral vein, 2D and 3D images of the MC-PROXYL distribution, but not C-PROXYL, were obtained. In the in vivo ESR experiment in mice intravenously treated with the spin probes, the observed 2D and 3D images indicated that MC-PROXYL distributed various regions of the head including brain. However, C-PROXYL did not. It is suggested that ESR-CT imaging system we improved could be a powerful tool to determine the reactions of free radicals and to monitor the free radical distribution and its metabolic reaction in living small animals.
Practice　： Dentistry
Keywords　： Electron spin resonance (ESR), Nitroxide spin probe, in vivo ESR imaging