アブストラクト（29巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Concentation-Effects of Nitric Oxide and Peroxynitrite on Leukocyte Superoxide Generation : The Role of Free Radicals on Inflammatory Response of Periondontal Disease.
Subtitle　： Selective Proceedings of 35th General Meeting of Kanagawa Odontological Society, 2000
Authors　： Chang-il Lee
Authors（kana）　：
Organization　： Department of Pharmacology, ESR Laboratory, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 29
Number　： 1
Page　： 36-38
Year/Month　： 2001 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： [Abstract] Activation of the NADPH oxidase-derived oxidant burst of polymorphonuclear leukocytes, PMNs, is of critical importance in in inflammatory disease including periodontitis. We directly measured the dose-dependent effects of NO・ and ONOO-on・O2-generation from human PMNs stimulated by phorbol myristate acetate using ESR spin trapping. Pretreatment with low physiological, μM, concentrations of NO・ from NO・ gas had no effect on PMN ・O2-generation, while high levels (>100μM) exerted inhibition. With ONOO-pretreatment, however, a biphasic modulation of ・O2-generation was seen with stimulation by μM levels but inhibition at higher levels. Thus, low physiological levels of NO・ present following PMN activation are converted to ONOO- which enhances ・O2- generation, while higher levels of NO・ or ONOO- feedback and inhibit ・O2- generation. This biphasic concentration-dependent regulation of the PMNs by NO・ derived ONOO- may be of critical importance in regulating the process of inflammation.
Practice　： Dentistry
Keywords　： Superoxide, Nitric oxide, Peroxynitrite, Electron spin resonance, Leukocyte, Inflammation