アブストラクト（30巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Expression of bcl-2 Family in Odontogenic Keratocysts and Dentigerous Cysts
Subtitle　： ORIGINAL ARTICLES
Authors　： Takayuki Yamamoto, Kentarou Suzuki, Atushi Nomura, Takuro Kanayasu, Sakae Akasaki, Yoshihisa Watanabe
Authors（kana）　：
Organization　： Department of Oral Pathology, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 30
Number　： 1
Page　： 9-15
Year/Month　： 2002 / 3
Article　： Original article
Publisher　： Kanagawa Odontological Society
Abstract　： Odontogenic keratocycts present an aggressive clinical course with a marked trend toward recurrence as compared with dentigerous cysts. Differences in the clinical behavior of cysts may be associated with apoptosis in the lining epithelium. In this study, we investigated the expression of apoptosis-related factors bcl-2, bcl-x, and bax in odontogenic keratocysts and dentigerous cysts by immunohistochemical analysis and reverse-transcription polymerase chain reaction (RT-PCR). Bcl-2 and bcl-xL were found to inhibit apoptosis, whereas bax and bcl-xS induced apoptosis. In odontogenic keratocycts, immunoreactivity for bcl-2 protein was detected in the basal and parabasal layers, while immunoreactivity for bcl-x and bax protein was detected in the suprabasal layers of the lining epithelium. Most dentigerous cysts, on the other hand, showed negative reactivity for bcl-2 protein. Immunoreactivity for bcl-x and bax protein was detected in all layers, including the basal layer of the lining epithelium of dentigerous cysts. RT-PCR analysis showed the presence of bcl-2, bcl-xL, and bax mRNA and the absence of bcl-xS mRNA in both types of cysts. Our results suggest that apoptosis in the lining epithelium is regulated by bcl-2, bcl-xL, and bax. In the basal layer of odontogenic keratocysts, bcl-2 protein, an inhibitor of apoptosis, was expressed, whereas bax protein, an stimulator of apoptosis, was not. Apoptosis activity in the basal layer of odontogenic keratocysts was apparently more strongly inhibited than that in the basal layer of dentigerous cysts. These results suggest that differences in apoptosis activity in the basal layer of the lining epithelium is one factor underlying the more aggressive clinical behavior of odontogenic keratocysts as compared with dentigerous cysts.
Practice　： Dentistry
Keywords　： Odontogenic keratocyst, Dentigerous cyst, Bcl-2, Bcl-x, Bax