アブストラクト（30巻2号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Development of cDNA Microarray for Analysis of Gene Expression Profiles during the Differentiation Process in the Craniomandibular System and for Molecular Diagnosis of Craniomandibular Disorders
Subtitle　： BKDC CLINICAL AND RESEARCH TOPICS : Research Topics from the KDC Research Center of Advanced Technology for Craniomandibular Function
Authors　： Kazuhito Izukuri1, Shigeyuki Ozawa1,2, Yojiro Maehata1, Shinji Takamizawa1,3, Eiro Kubota2,4, Sadao Sato3,4, Ryu-Ichiro Hata1,4
Authors（kana）　：
Organization　： 1Department of Biochemistry and Molecular Biology, Kanagawa Dental College, 2Department of Oral Surgery, Kanagawa Dental College, 3Department of Orthodontics, Kanagawa Dental College, 4Research Center of Advanced Technology for Craniomandibular Function, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 30
Number　： 2
Page　： 137-140
Year/Month　： 2002 / 9
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： Two primary factors are responsible for the significant transition in recent biological and biomedical research: the massive increase in the amount of DNA sequence information and the development of technologies to exploit its use. Functional genomics is one of the new fields of biological science in the post human genome project era and DNA microarray or DNA chip method is one representative technology to make use of these recent discoveries. For a routine analysis of changes in the gene expression profiles of tissue and cells of interest, it is necessary to develop a specific DNA chip and an adequate method for processing the data obtained by scanning it. Herein we describe the basic principle of DNA chip methodology and give an example of a system that we are currently developing for the clarification of gene expression profiles during normal differentiation and for the diagnosis of pathological conditions in the craniomandibular system.
Practice　： Dentistry
Keywords　： cDNA microarray, DNA chip, cDNA library, Craniomandibular system