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アブストラクト（30巻1号：The Bulletin of Kanagawa Dental College）
English
| Title ： | Purification of CAP from bovine developing tooth germ at root forming stage |
|---|---|
| Subtitle ： | Selective Proceedings of 36th General Meeting of Kanagawa Odontological Society, 2001 |
| Authors ： | Shintaro Hattori1, Masahiro Saito2, Toshio Teranaka2, Minoru Toyoda1 |
| Authors（kana） ： | |
| Organization ： | 1Department of Prothodontics, Kanagawa Dental College, 2Department of Operative Dentistry and Endodontics, Kanagawa Dental College, 3Institute for frontier oral science, Kanagawa Dental College |
| Journal ： | The Bulletin of Kanagawa Dental College |
| Volume ： | 30 |
| Number ： | 1 |
| Page ： | 79-80 |
| Year/Month ： | 2002 / 3 |
| Article ： | Report |
| Publisher ： | Kanagawa Odontological Society |
| Abstract ： | Cementum-derived attachment protein (CAP) has been reported as a collagenous attachment protein existed in cementum. Recently, we have shown that the CAP was a putative cementoblasts marker in developing tooth germ, and it may contribute cementogenesis through the promotion of cell attachment to dental follicle cells. To identify the molecular structure of CAP, it was extracted from bovine tooth germ, and separated by diethylamino ethyl (DEAE)-cellulose chromatography and Q-Sepharose fast flow column, and purified by Mono Q high performance liquid chromatography (HPLC). The purified preparation contained single band migrating with molecular mass of 65kDa. The 65kDa protein was positive for anti-CAP monoclonal antibody, and it was susceptible to bacterial collagenase. These data indicated that CAP was able to purify from bovine developing tooth germ. |
| Practice ： | Dentistry |
| Keywords ： | cementum, chromatography, cementum-derived attachment protein, monoclonal antibody |
