アブストラクト（30巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Preparation of Recombinant Human Bone Sialoprotein Using Baculovirus Expression System
Subtitle　： Selective Proceedings of 36th General Meeting of Kanagawa Odontological Society, 2001
Authors　： Akira Tsunoda1, Masahiro Saito1, Keisuke Handa1, Masato Yamauchi2, Miwa Horiguchi2, Sadao Sato2, Toshio Teranaka1
Authors（kana）　：
Organization　： 1Department of Operative Dentistry and Endodontics, Kanagawa Dental College, 2Department of Orthodontics, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 30
Number　： 1
Page　： 81-83
Year/Month　： 2002 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： Bone sialoprotein (BSP) is a phosphorylated glycoprotein found in the mineralized matrix. Being in the mineralized matrix such as bone, dentin and cementum, it supports the cell attachment through an integrin receptor. During cementogenesis, it is expressed in cementoblasts, and interaction between the cementoblasts and BSP may be critical for cementum formation. However underlying mechanisms by which BSP controls cementogenesis are not fully understood yet. In order to elucidate the physiological role of BSP during cementogenesis, we produced recombinant human BSP (rhBSP). rhBSP with His, fusion peptide was expressed in insect cells using a baculovirus expression system, and purified by metal-affinity column chromatography. Purified rhBSP migrated as discrete bands of 60 kDa and 65 kDa in an SDS PAGE and Western blotting. Bovine dental follicle cells, which contain cementoblast progenitors, adhered to the dish surface when it was coated by rhBSP. These results indicate that the baculovirus expression system is useful in producing functional rhBSP.
Practice　： Dentistry
Keywords　： Bone sialoprotein, Baculovirus, Cementogenesis