アブストラクト（31巻1号：The Bulletin of Kanagawa Dental College）

The Bulletin of Kanagawa Dental College

English

Title　： Vitamin D regulates human osteoblast differentiation by stimulating type I collagen synthesis
Subtitle　： Selective Proceedings of 37th General Meeting of Kanagawa Odontological Society, 2002
Authors　： Yojiro Maehata1, Shinji Takamizawa2, Shigeyuki Ozawa3, Kazuhito Izukuri1, Yasumasa Kato1, Sadao Sato2,4, Eiro Kubota3,4, Ryu-Ichiro Hata1,4
Authors（kana）　：
Organization　： 1Department of Biochemistry and Molecular Biology, Kanagawa Dental College, 2Department of Craniofacial Growth and Development Dentistry, Kanagawa Dental College, 3Department of Oral Surgery, Kanagawa Dental College, 4Research Center of Advanced Technology for Craniomandibular Function, Kanagawa Dental College
Journal　： The Bulletin of Kanagawa Dental College
Volume　： 31
Number　： 1
Page　： 77-80
Year/Month　： 2003 / 3
Article　： Report
Publisher　： Kanagawa Odontological Society
Abstract　： In addition to its structural function, type I collagen has a role as a regulator of proliferation and differentiation by transmitting signals via binding with integrin receptors on the cell membrane. In order to investigate the mechanism of osteoblast differentiation, we cultured MG-63 cells, which are human osteoblast-like cells, in the absence or presence of L-ascorbic acid 2-phosphate (Asc 2-P) and/or 1α, 25 (OH)2 vitamin D3 (VD3). Cell growth as observed by cell number and DNA content was increased by the presence of Asc 2-P in the culture medium. Collagen synthesis and alkaline phosphatase (ALP) activity were also stimulated by the presence of Asc 2-P as well as by that of VD3. The co-presence of Asc 2-P and VD3 had a synergistic effect on the collagen synthesis and ALP activity of the cells. Inhibition of collagen synthesis by the presence of inhibitors of collagen synthesis attenuated the stimulative effect of Asc 2-P on ALP activity. Gene expressions of COL1A1, COL1A2, and osteocalcin were increased by the presence of VD3: and the expression of a new transcription factor, "osterix" was observed as well. These results indicate that Asc 2-P and VD3 signals have synergistic effect on the differentiation of human osteoblast-like MG-63 cells and that collagen synthesis is essential for VD3 to express its full activity toward osteoblast differentiation.
Practice　： Dentistry
Keywords　： Osteoblast, Collagen, 1α,25 (OH)2 vitamin D3, Osterix